Highway 24 was closed near Kossuth Road for part of Monday morning following a crash that sent one-person to hospital.
The crash occurred around 9 a.m., and prompted a closure in both directions south of Kossuth.
It involved a sedan and a box truck.
Waterloo Regional Police say the driver of the sedan was taken to hospital with unknown injuries, while the driver of the truck was not hurt.
Police continue to investigate the crash.