Using pitchforks, investigators removed large amounts of straw Tuesday from the barn on the Roseville Road farm belonging to Dellen Millard.
Earlier in the day, Waterloo Regional Police officers dropped off at the site what appeared to be construction supplies, including sheets of plywood.
It was the second straight day of police activity at the farm in connection with the July 2012 disappearance of Toronto woman Laura Babcock.
Police have said that Babcock, 23, is romantically linked to the 28-year-old Millard, who is charged with first-degree murder in the death of Tim Bosma.
Bosma’s burnt remains were found at the same North Dumfries farm following an extensive police search in late May.
He was last seen leaving his Ancaster home with two men who had asked to test-drive a vehicle he was selling.
Mark Smich, 25, is also charged with first-degree murder in his death.
Police returned to the farm in early June in connection with Babcock’s disappearance, but did not find anything at that time.
Also at the farm Tuesday was Thomas Ryan, Babcock’s uncle.
Ryan told reporters he wanted to see the farm and police activity for himself after learning of Monday’s events.
“When your niece is on the news, front page, it’s difficult to go to work,” he said.
Ryan called Babcock a “happy, beautiful, intelligent woman” who he last spoke with about two weeks before her disappearance.
“The last thing we knew, she was out looking for someplace new to live and left home on a fairly positive note,” he said.
Police say they are not searching any other locations in connection with the case.